General Information

Event name: PT-03-002
Country: PORTUGAL
Nature of the harmful event:
Seafood toxins
Event directly affected:
Humans
Toxicity detected: Yes (Approximate range: 0.4 - 420 µg/100g OA eq.)
Associated syndrome: DSP
Unexplained toxicity: No
Species implicated in toxin transmission (transvector): Mytilus edulis (blue mussels), Solen marginatus (razor clams), other mollusc bivalves.
Report the outcome of a monitoring programme: Yes (HAB Monitoring programme.)
Event occurred before in this location: Yes
Individuals to contact: VILARINHO, Mª da Graça , VALE, Paulo

Location & Date

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Location: Latitude: 36.500000, Longitude: -7.000000
General location information: Whole coast of Portugal., Mainly NW coast of Portugal.
HAB Area code(s): PT-01 PT-02 PT-03 PT-04 PT-05 PT-06 PT-07
Additional location information: 37º - 42ºN; 7º - 10ºW. 600 Km of shoreline. Several events during the above period with a higher incidence along the NW coast. (i.e. Between Lisbon/Setubal bays and Porto).
Bloom event dates (yyyy/mm/dd): Start: 2003-06-17, End: 2003-12-01
Quarantine levels dates (yyyy/mm/dd):
Additional date-related information:

Microalgae

Causative organism known: Yes
Causative Species/Genus: Dinophysis acuminata (21,800 cells/L)
During the peak of July 2003 (Porto).
Dinophysis acuta (101,200 cells/L)
During the peak of September 2003 (Aveiro).
Co-Ocurring Species/Genus: Cylindrotheca closterium (7,500 cells/L)
Prorocentrum micans ( cells/L)
Gymnodinium sp. (3,000 cells/L)
Dinophysis caudata (4,000 cells/L)
Dinophysis dens (5,000 cells/L)
Chaetoceros spp. (57,000 cells/L)
During peak of D. Acuminata
Leptocylindrus danicus (4,000 cells/L)
Guinardia delicatula (2,000 cells/L)
Leptocylindrus danicus (13,000 cells/L)
During peak of D. Acuta
Chlorophyll concentration, if known: 4 µg/L Chlª during D. acuta peak. µg/l
Additional bloom information:
Event-related bibliography:

Environmental Conditions

Weather: Calm Turbidity (NTU): Wind direction:
Stratified water: Yes Oxygen content (nL/L): Wind velocity:
Temperature (°C): 16.8 Oxygen saturation %: Current Direction:
Sechhi disk (m): Salinity: 35.4 Current Velocity: 0
Nutrient information:
Temperature Range During Event: Max: 19 °C, Min: 15 °C
Salinity Range During Event: Max: 35, Min: 34
Bloom location in the water column:
Subsurface
Growth: Advected
In situ
Growth Comments
Additional Environmental information:

Toxin Assay Information

Species containing the toxin Toxin type Toxin details Max. concentration Assay type
Mytilus sp., Solen sp. & other molluscs bival Okadaic Acid OA eq 420 µg/100g ELISA
Mytilus sp., Solen sp. & other molluscs bival Okadaic Acid OA eq 420 µg/100g LC-MS
Kit used: No Type of kit used:
Additional information: Assay for OA and DTX2 recovered after alkaline hydrolisis, either by LC-MS or DSP-check inmmunoassay kit. OA, DTX2, acyl ester OA and DTX2 detected.
Economic losses:
Management decision: Close the area to bivalve catch.
Additional harmful effect information: ND (not detected) <0.4 ug/g 100 g OA equiv.
Checked at 05:33 on 22 Feb 2018