General Information

Event name: IND-15-001
Country: INDIA
Nature of the harmful event:
Seafood toxins
Event directly affected:
Humans
Toxicity detected: Yes (Approximate range: Ciguatera toxin)
Associated syndrome: CFP (Ciguatera Fish Poisoning)
Unexplained toxicity: No
Species implicated in toxin transmission (transvector): Lutjanus bohar
Report the outcome of a monitoring programme: No
Event occurred before in this location: No
Individuals to contact: Dr K B PADMAKUMAR

Location & Date

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Location: Latitude: , Longitude:
General location information: Mangalore, South India
HAB Area code(s): IN_01
Additional location information:
Bloom event dates (yyyy/mm/dd): Event Date:2015-06-08
Quarantine levels dates (yyyy/mm/dd):
Additional date-related information:

Microalgae

Causative organism known: No
Causative Species/Genus:
Co-Ocurring Species/Genus:
Chlorophyll concentration, if known: µg/l
Additional bloom information:
Event-related bibliography: Presumptive case of ciguatera fish poisoning in Mangalore, India Moger Rajeish, Malathi Shekar, Hoovinahalli Nataraju Madhushree and Moleyur Nagarajappa Venugopal*CURRENT SCIENCE, VOL. 111, NO. 9, 10 NOVEMBER 2016

Environmental Conditions

Weather: Turbidity (NTU): Wind direction:
Stratified water: Oxygen content (nL/L): Wind velocity:
Temperature (°C): Oxygen saturation %: Current Direction:
Sechhi disk (m): Salinity: Current Velocity:
Nutrient information:
Temperature Range During Event: Max: °C, Min: °C
Salinity Range During Event:
Bloom location in the water column:
Growth:
Growth Comments Environmental parameters not mentioned
Additional Environmental information:

Toxin Assay Information

Species containing the toxin Toxin type Toxin details Max. concentration Assay type
Kit used: Type of kit used:
Additional information: The toxin extract was tested for potential presence of CTXs and its toxicity by mouse bioassay as previously described21. Briefly, BALB/c mice weighing 1822 g were obtained and housed in a controlled-environment at ~25C and fed with food and water. Mouse bioassay was performed using a portion (~20 mg/mouse) of the diethyl ether fraction. The fraction was suspended in 0.5 ml 1% Tween 60/0.9% saline, heated at 37C until dissolved, cooled and injected intra-peritoneally into healthy mice in duplicates. Control mice were administered with 0.5 ml of 1% Tween 60/0.9% saline. The mice were observed for symptoms of CTX poisoning and their survival time recorded. The relationship between dose and death time was used to quantify each fraction. The quantity of CTX present was calculated by: log MU = 2.3log(1 + T 1), where MU = number of mouse units of CTX injected and T = time to death in hours. One MU is the lethal dose for a 20 g mouse, which is equivalent to 5 ng CTX-122.
Economic losses:
Management decision:
Additional harmful effect information:
Created at 09:56 on 29 Nov 2018
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