| Event name: | IND-15-001 | |
| Country: | INDIA | |
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Nature of the harmful event: |
Seafood toxins | |
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Event directly affected: |
Humans | |
| Toxicity detected: | Yes (Approximate range: Ciguatera toxin) | |
| Associated syndrome: | CFP (Ciguatera Fish Poisoning) | |
| Unexplained toxicity: | No | |
| Species implicated in toxin transmission (transvector): | Lutjanus bohar | |
| Report the outcome of a monitoring programme: | No | |
| Event occurred before in this location: | No | |
| Individuals to contact: | Dr K B PADMAKUMAR | |
| Location: | Latitude: , Longitude: | |
| General location information: |
Mangalore, South India HAB Area code(s): IN-01 |
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| Additional location information: | ||
| Bloom event dates (yyyy/mm/dd): |
Event Date:2015-06-08 |
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| Causative organism known: | No | |
| Causative Species/Genus: | ||
| Co-Ocurring Species/Genus: | ||
| Chlorophyll concentration, if known: | µg/l | |
| Additional bloom information: | ||
| Event-related bibliography: | Presumptive case of ciguatera fish poisoning in Mangalore, India Moger Rajeish, Malathi Shekar, Hoovinahalli Nataraju Madhushree and Moleyur Nagarajappa Venugopal*CURRENT SCIENCE, VOL. 111, NO. 9, 10 NOVEMBER 2016 | |
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| Temperature Range During Event: | Max: °C, Min: °C | |||||||||||||||||||||||||
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| Bloom location in the water column: | ||||||||||||||||||||||||||
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| Growth Comments | Environmental parameters not mentioned | |||||||||||||||||||||||||
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| Additional information: | The toxin extract was tested for potential presence of CTXs and its toxicity by mouse bioassay as previously described21. Briefly, BALB/c mice weighing 18–22 g were obtained and housed in a controlled-environment at ~25C and fed with food and water. Mouse bioassay was performed using a portion (~20 mg/mouse) of the diethyl ether fraction. The fraction was suspended in 0.5 ml 1% Tween 60/0.9% saline, heated at 37C until dissolved, cooled and injected intra-peritoneally into healthy mice in duplicates. Control mice were administered with 0.5 ml of 1% Tween 60/0.9% saline. The mice were observed for symptoms of CTX poisoning and their survival time recorded. The relationship between dose and death time was used to quantify each fraction. The quantity of CTX present was calculated by: log MU = 2.3log(1 + T – 1), where MU = number of mouse units of CTX injected and T = time to death in hours. One MU is the lethal dose for a 20 g mouse, which is equivalent to 5 ng CTX-122. | ||||||
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